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Microbial community analysis was performed based on the 16S rRNA gene. The results obtained for the archaea were similar to those of the 16S rRNA gene analysis, indicating that most of the archaeal community members are members of the domain Euryarchaeota. The phylogenetic group ArcI was not detected. This group is described as associated with cold habitats, such as Antarctica ([@B41]), and was considered a possible contributor to the biosynthesis of the nanomaterial for treatment of skin wounds ([@B41], [@B42]). The phylogenetic groups ArcII and ArcIII were detected and represented 8.51% and 1.57% of all sequences, respectively, with *L. lactis* subsp. *cremoris* as the dominant member. These results are similar to those reported for the dairy environment ([@B43]).
The results of the analysis of the bacterial community indicate that *P. acnes* and *S. epidermidis* were the two main members of the skin surface microbiome. The highest number of sequences were affiliated to the genus *Pseudomonas* (11.28%), followed by *Staphylococcus* (10.01%) and *Micrococcus* (2.61%), which contributed relatively low amounts of sequences. The presence of these species is commonly associated with skin, and their presence in the same proportion suggests that they are probably more important in terms of their abundance than the number of sequences. Other species found in relative high amounts were *Propionibacterium* (6.29%), which is an anaerobic bacteria and is considered a metabolically versatile species, capable of fermenting a variety of sugars and producing hydrogen ([@B44]). In this study, the human skin microbiota was analysed from a region of the body that is highly exposed to microorganisms, which could explain the high abundance of bacterial species.
In general, the core microbiomes were shared by the different individuals included in the study. However
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[Transformation of a main bile acid deoxycholic acid into lithocholic acid in vitro].
To study the metabolism of an important bile acid deoxycholic acid(DCA) into lithocholic acid (LCA) in vitro, and to research the effect of the bile acid cholylglycine on the metabolism of DCA into LCA. The metabolic experiments were performed in vitro. DCA was first hydrolyzed into three products, cholylglycine, deoxycholic acid, and glycine, in duodenum and jejunum of SD rats in vivo and in vitro. Then these products were further metabolized into LCA and ursodeoxycholic acid (UDCA) in rat intestinal microsomes and in duodenum of SD rats in vivo and in vitro. The bile acid cholylglycine was detected in serum and urine of the SD rats after the administration of DCA. The content of cholylglycine increased and reached the peak value in serum and urine of the rats within 6 hours after the intravenous administration of DCA (P < 0.01). The lithocholic acid was detected in bile of rats. The content of LCA in bile of the rats with DCA treatment increased significantly and reached the peak value within 3